Characterization ofPhytophthora nicotianaeisolates in southeast Spain and their detection and quantification through a real-time TaqMan PCR
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چکیده
منابع مشابه
A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane
Sporisorium scitamineum is a fungal smut pathogen epidemic in sugarcane producing areas. Early detection and proper identification of the smut are an essential requirement in its management practice. In this study, we developed a TaqMan real-time PCR assay using specific primers (bEQ-F/bEQ-R) and a TaqMan probe (bEQ-P) which were designed based on the bE (b East mating type) gene (Genbank Acces...
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Noroviruses (NoVs) are the most commonly identified cause of outbreaks and sporadic cases of acute gastroenteritis. We evaluated and optimized NoV-specific TaqMan real-time reverse transcription (RT)-PCR assays for the rapid detection and typing of NoV strains belonging to genogroups GI and GII and adapted them to the LightCycler platform. We expanded the detection ability of the assays by deve...
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ژورنال
عنوان ژورنال: Journal of the Science of Food and Agriculture
سال: 2015
ISSN: 0022-5142
DOI: 10.1002/jsfa.7300